Lipsae脂肪酶 质谱级

 

订货号： HLS LIP001C

 

说明: 脂肪酶可催化水溶液中三酰甘油的水解，生成甘油和游离脂肪酸。该脂肪酶来源于猪胰腺。在PBS缓冲液或Tris-HCL缓冲液中，Triglyceride + H2O  －> Diglyceride + Fatty Acid。

 

物理形态:   冻干粉

分子量:      48kDa.

溶解液：   Lipase 脂肪酶用100ul  5mM氯化钙溶解(使用前冰浴）

储存条件：冻干粉末存储在-20℃冰箱，再溶解的酶储存在-20℃或- 80℃

保质期：    24月在-20℃

pH 值范围: 在 PH=7.7 时， Lipase脂肪酶具有最佳活性

适用范围： Lipase脂肪酶特异性水解血清或动物组织样品中的三酰甘油

溶液酶解SOP（仅供参考）:

20ul 血清样本加20ul 100mM PBS buffer稀释PH=7.7， 37℃保温30min，立即加入2-6ul Lipase 脂肪酶保温15min

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Part No.                      Name                Size

HLS LIP001C            Lipase                50 μg

 

Description: Lipase catalyzes the hydrolysis of triacylglycerols in aqueous solutions to generate glycerol and free fatty acids. The lipase is derived from porcine pancreas.

Physical Appearance: Lyophilized powder.

Molecular Weight: 48 kDa

Resuspension Buffer: Dissolve with 100 μL of 5 mM calcium chloride (ice bath before use).

Storage Conditions: Powder at -20 °C.

Shelf Life: 12 months at -20 °C

pH Range: Maximally active at pH 7.7.

Application: Lipase specifically hydrolyzes triacylglycerols in serum or animal tissue samples

In-Solution Protein Digestion Protocol (Recommended):

Add 20 μL of 100 mM PBS buffer into 20 μL serum sample and adjust to pH 7.7. Incubate samples at 37 ℃ for 30 min. Then, immediately add 2 - 6 μL lipase and incubate for 15min.

Activity: 20000 U/mg.

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