N-Glycan聚糖分子标签／N-Glycan Label Reagent
N-Glycan 聚糖标签: N_Glycan Label Reagent
用于N-linked糖蛋白（包括抗体)经过PNGase F糖苷酶酶切后溶液中聚糖的超快速标记。 标记后的聚糖具有灵敏的荧光和质谱离子化特性。使用LC-FLR or LC-MS的定量分析检测。该试剂独特的化学属性提供了更高的荧光定量和最高的质谱响应。
产品用途：
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生物治疗蛋白N-聚糖表征分析
产品特点：
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质谱级干粉试剂
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标记效率高：3min
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热稳定性好：24小时
超快速30min标记流程：
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取 10ul. 1.5 ug/ul mAb 脱盐样品
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加 10ul 3% BT Surfactant w/5mM TCEP 变性还原缓冲液
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干浴 95°C 加热3min； 冷却至室温
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加 10µl 1.5 unit/ul rPNGase F糖苷酶， 干浴50°C 孵育15min
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加 10ul 82 ug/ul N-Glycan Label标签试剂， 室温反应5min
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加 15ul 无水DMF； 加45ul 乙腈
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LC-MS分析
for ultrafast labeling of N-glycans in solution after PNGase F glycosidase cleavage of N-linked glycoproteins (including antibodies).Labeled N-glycans have sensitive fluorescence and mass spectrometry ionization properties and quantitative or identification analytical detection by LC-FLR or LC-MS. The unique chemical properties of this reagent provide higher fluorescence quantification and the highest mass spectral response.
Application:
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Characterization of Biotherapeutic Protein N-Glycans
the key Featuers ：
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Mass spectrometry grade dry powder reagents
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High labeled efficiency: 3min
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Good thermal stability: 24 hours